tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci
نویسندگان
چکیده
منابع مشابه
tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci
Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orientations to which (single-strand) oligos rather than (double-stranded) adaptors are ligated. This s...
متن کاملCorrection: A Next-Generation Sequencing Method for Genotyping-by-Sequencing of Highly Heterozygous Autotetraploid Potato
Assessment of genomic DNA sequence variation and genotype calling in autotetraploids implies the ability to distinguish among five possible alternative allele copy number states. This study demonstrates the accuracy of genotyping-by-sequencing (GBS) of a large collection of autotetraploid potato cultivars using next-generation sequencing. It is still costly to reach sufficient read depths on a ...
متن کاملGenotyping-by-Sequencing in Plants
The advent of next-generation DNA sequencing (NGS) technologies has led to the development of rapid genome-wide Single Nucleotide Polymorphism (SNP) detection applications in various plant species. Recent improvements in sequencing throughput combined with an overall decrease in costs per gigabase of sequence is allowing NGS to be applied to not only the evaluation of small subsets of parental ...
متن کاملComparing Genotyping-by-Sequencing and Single Nucleotide Polymorphism Chip Genotyping for Quantitative Trait Loci Mapping in Wheat
Arrayor chip-based single nucleotide polymorphism (SNP) markers are widely used in genomic studies because of their abundance in a genome and lower cost per data point than older marker technologies. Genotyping-bysequencing (GBS), a relatively newer approach of genotyping, suggests equal appeal because of its lesser cost per data point and the avoidance of ascertainment bias during genotyping. ...
متن کاملSorting duplicated loci disentangles complexities of polyploid genomes masked by genotyping by sequencing.
Many plants and animals of polyploid origin are currently enjoying a genomics explosion enabled by modern sequencing and genotyping technologies. However, routine filtering of duplicated loci in most studies using genotyping by sequencing introduces an unacceptable, but often overlooked, bias when detecting selection. Retained duplicates from ancient whole-genome duplications (WGDs) may be foun...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2017
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkx853